Secondary Appointments

Department of
Biological Chemistry

In addition to primary faculty, the department of biological chemistry is comprised of faculty from various departments throughout Johns Hopkins. Faculty who hold a secondary appointment in the department add yet another layer to the intellectual environment, scientifically and socially. Together, we continue to make significant contributions to our success. The inclusion of secondary faculty into our department further illustrates our commitment to diversity of scientific approach and the broad interest at Ho pkins in our departmental endeavors.

Peter Agre, M.D.
pagre@jhu.edu
443-287-8745

Directs the Johns Hopkins Malaria Research Institute: lab studies in Baltimore and field work in Africa

Peter Devreotes, Ph.D.
pnd@jhmi.edu
410-955-3225

Genetic analysis of chemotaxis eukaryotic cells

Peter Devreotes
Professor and Director of Cell Biology
Johns Hopkins University School of Medicine

725 N. Wolfe St., 114 WBSB
Baltimore, MD 21205

Email: pnd@jhmi.edu
Office Phone: 410-614-9461
Lab Phone: 410-955-4699

Visit my Lab Page

Program Description

Chemotaxis plays a key role in immune response, wound healing, angiogenesis, and embryogenesis as well as mediating cancer metastasis. Research in Dictyostelium discoideum has shown that chemoattractants are sensed by GPCRs and rapidly trigger a unique network of signaling pathways. Our strategy is to use the genetics of Dictyostelium to discover mechanisms by which cells sense chemical gradients and to apply this information to other eukaryotic cells such as human neutrophils and epithelial cells. We found that cell motility depends on spontaneous activation of the signal transduction network and that directional cues bias the threshold for activation. Numerous signal transduction events are activated or inactivated on each pseudopod. For example, activation of PI3K and inactivation of PTEN leads to a local accumulation of PIP3. Activation of protein kinases B requires both PIP3 and components of the Tor complex 2. By screening gene-tagged cell lines for mutants with phenotypes similar to those lacking the known signal transduction components, we are identifying novel genes involved in pathways.

Recent Publications

Swaney K.F., Borleis J., Iglesias P.A., and Devreotes, P.N. 2015. Novel protein Callipygian defines the back of migrating cells. Proc Natl Acad Sci U S A. Jun 30. Pii:201509098 [Epub ahead of print]. PMID: 26130809.
PubMed Reference

Cai H, Katoh-Kurasawa M., Muramoto T, Santhanam B, Long Y, Li L, Ueda M, Iglesias PA, Shaulsky G, and Devreotes PN. 2013. Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer. Science. 2014 Mar 21;343(6177):1249531. Doi: 10.1126/science.1249531. PMCID: PMC Journal

PubMed Reference

Huang C,Tang M, Shi C, Iglesias P, and Devreotes PN. An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration. Nat Cell Biol. 2013 Nov;15(11):1307-16.

PubMed Reference

Nguyen HN, Afkari Y, Senoo H, Sesaki H, Devreotes PN, and Iijima M. Mechanisms of human PTEN localization revealed by heterologous expression in Dictyostelium. Oncogene, in press.

Tang M, Iijima M, and Devreotes PN. 2011. Generation of cells that ignore the effects of PIP3 on cytoskeleton. Cell Cycle, 10:2817-2818.

PubMed Reference

Tang M, Iijima M, Kamimura Y, Chen L, Long Y, and Devreotes PN. 2011 Disruption of PKB signaling restores polarity to cells lacking tumor suppressor PTEN. Mol. Biol. Cell. 22:437-447.

PubMed Reference

Swaney KF, Huang CH, Devreotes PN. 2010 Eukaryotic Chemotaxis: A network of signaling pathways controls motility, directional sensing, and polarity. Annu Rev Biophys 278:20445-20448.

PubMed Reference

Xiong Y, Huang CH, Iglesias PA., and Devreotes PN. 2010 Cells navigate with a local-excitation, global-inhibition-biased excitable network. PNAS, 107:17079-17086.

PubMed Reference

Rahdar M, Inoue T, Meyer T, Zhang J, Vazquez F, Devreotes PN. 2008 A phosphorylation-dependent intramolecular interaction regulates the membrane association and activity of the tumor suppressor PTEN. PNAS 106:480-485.

PubMed Reference

Chen L, Iijima M, Tang M, Landree MA, Huang YE, Xiong Y, Iglesias PA, and Devreotes PN. 2007 PLA2 and PI3K/PTEN pathways act in parallel to mediate chemotaxis. Dev. Cell 12(4):603-614.

PubMed Reference

Janetopoulos C, Borleis J, Vazquez F, Iijima M, and Devreotes PN. 2005 Temporal and spatial regulation of phosphoinositide signaling mediates cytokinesis. Dev. Cell 8:467-477.

PubMed Reference

Iijima M, Huang YE, Luo HR, Vazquez F, and Devreotes PN. 2004 Novel mechanism of PTEN regulation by its Phosphatidylinositol 4,5-bisphosphate binding motif is critical for chemotaxis. J. Biol. Chem. 279(16):16606-16613.

PubMed Reference

Luo, HR, Huang YE, Chen JC, Saiardi A, Iijima M, Ye K, Huang Y, Nagata E, Devreotes PN, and Snyder SH. 2003 Inositol pyrophosphates mediate chemotaxis in Dictyostelium via pleckstrin homology domain-PtdIns(3,4,5)P3 interactions. Cell 114(5), 559-572.

PubMed Reference

Iijima M, Huang E, and Devreotes PN. 2002 Temporal and spatial regulation of chemotaxis. Dev. Cell 3, 469-478.

PubMed Reference

Janetopoulos C, Jin T and Devreotes PN. 2001. Receptor mediated activation of heterotrimeric G-proteins in living cells. Science, 291, 2408-2411.
PubMed Reference

Parent C. and Devreotes PN. 1999. A Cell's Sense of Direction. Science, 284, 765-770.
PubMed Reference

Parent C, Blacklock B, Froelich W, Murphy D and Devreotes PN. 1998. G protein signaling events are activated at the leading edge of chemotactic cells. Cell, 95, 81-91.
PubMed Reference

Pitt GS, Milona N, Borleis J, Lin KC, Reed RR and Devreotes PN. 1992. Structurally distinct and stage-specific adenylyl cyclase genes play different roles in Dictyosteilum development. Cell 69, 305-315.
PubMed Reference

Klein PS, Sun TL. Saxe CL III, Kimmel AR, Johnson RL and Devreotes PN. 1988. A chemoattractant receptor controls development in Dictyostelium discoideum. Science 241, 1467-1472.
PubMed Reference

Tomchik KJ and Devreotes PN. 1981. Cyclic AMP waves in Dictyostelium discoideum: A demonstration by isotope dilution fluorography. Science 212, 443-446.
PubMed Reference

Yolanda M. Fortenberry photo unavailable

Yolanda M. Fortenberry, Ph.D.
yforten1@jhmi.edu
410-614-0285

Molecular mapping, Biochemistry, Regulation of tumor cells, Thrombosis, Hemostasis

Richard Huganir, Ph.D.
rhuganir@jhmi.edu
410-955-4050

Regulation of Neurotransmitter Receptors and Brain Function in Health and Disease

Richard Huganir
Professor and Director of Neuroscience
Johns Hopkins University School of Medicine

725 N. Wolfe St.
Baltimore, MD 21205

Email: rhuganir@jhmi.edu
Office Phone: (410) 955-0877
Lab Phone:

Visit my Lab Page

Program Description

Neurotransmitter receptors mediate signal transduction at the postsynaptic membrane of synaptic connections between neurons in the nervous system. We have been studying the molecular mechanisms in the regulation of neurotransmitter receptor function. Recently we have focused on glutamate receptors, the major excitatory receptors in the brain. Glutamate receptors can be divided into two major classes: AMPA and NMDA receptors. AMPA receptors mediate rapid excitatory synaptic transmission while NMDA receptors play important roles in neuronal plasticity and development. Studies in our laboratory have found that both AMPA and NMDA receptors are multiply phosphorylated by a variety of protein kinases. Phosphorylation regulates several functional properties of these receptors including conductance and membrane targeting. Recent studies in our lab have demonstrated that the phosphorylation of AMPA receptors is regulated during cellular models of learning and memory such as long-term potentiation (LTP) and long-term depression (LTD). Moreover, phosphorylation of the AMPA receptor GluR1 subunit is required for the expression of these forms of plasticity and for the retention of spatial memory and also regulates emotional memory formation and erasure.

We have also been examining the mechanisms of the subcellular targeting and clustering of glutamate receptors at synapses. We have recently identified a variety of proteins that directly or indirectly interact with AMPA and NMDA receptors. We have found a novel family of proteins that we call GRIPs (Glutamate Receptor Interacting Proteins) that directly bind to the C-termini of the GluR2/3 subunits of AMPA receptors. GRIPs contain seven PDZ domains, protein-protein interaction motifs, which crosslink AMPA receptors to each other or link them to other proteins. In addition, we have found that the C-termini of GluR2 also interacts with the PDZ domain of PICK1, a protein kinase C-binding protein that is found at excitatory synapses. The GluR2 subunit also interacts with the NSF protein, a protein involved in the regulation of membrane fusion events. These AMPA receptor interacting proteins are critical in the proper membrane trafficking and synaptic targeting of these receptors. We have shown that the binding of PICK1 and GRIP is required for a specific form of LTD in the cerebellum that is a cellular model for motor learning. Moreover, we have found that this receptor complex is critical for hippocampal LTP and LTD and spatial learning.

In addition to these studies on AMPA receptors, we have been characterizing a separate NMDA receptor associated protein complex that is important in synaptic targeting and downstream signaling of NMDA receptors. We have identified an excitatory synapse specific rasGAP, which we call synGAP that regulates synaptic Ras signaling and has profound effects on synaptic plasticity.

Importantly, recent evidence has implicated glutamate receptor associated complexes in several neurological and psychiatric disorders including Alzheimer’s disease, schizophrenia, autism, mental retardation as well as in chronic pain and drug addiction.

In summary, we have examined the molecular mechanisms underlying the regulation of neurotransmitter receptor function. Our studies have suggested that regulation of receptor function may be a major mechanism for the regulation of synaptic plasticity in the nervous system in health and disease and may be an important determinant of animal behavior.

Recent Publications

Volk LJ, Bachman JL, Johnson R, Yu Y, Huganir,RL. (2013) PKMz is not required for hippocampal synaptic plasticity, learning and memory. Nature. 493(7432): 420-3.
PubMed Reference

Thomas GM, Hayashi T, Chiu SL, Chen CM, Huganir RL. (2012) Palmitoylation by DHHC5/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking. Neuron. 73(3):482-96.
PubMed Reference

Makuch L, Volk L, Anggono V, Johnson RC, Yu Y, Duning K, Kremerskothen J, Xia J, Takamiya K, Huganir RL. (2011) Regulation of AMPA receptor function by the human memory-associated gene KIBRA. Neuron. 71(6): 1022-9.
PubMed Reference

Makino, Y., Johnson, R.C., Yu, Y., Takamiya, K., Huganir, R.L. (2011) Enhanced synaptic plasticity in mice with phosphomimetic mutation of the GluA1 AMPA receptor. Proc Natl Acad Sci U S A. 108(20):8450-5.
PubMed Reference

Mejias, R., Adamczyk, A., Anggono, V., Niranjan, T., Thomas, G.M., Sharma, K., Skinner, C., Schwartz, C.E., Stevenson, R.E., Fallin, M.D., Kaufmann, W., Pletnikov, M., Valle, D., Huganir, R.L., Wang, T. (2011) Gain-of-function glutamate receptor interacting protein 1 variants alter GluA2 recycling and surface distribution in patients with autism. Proc Natl Acad Sci USA. 108(12):4920-5.
PubMed Reference

Clem R, Huganir RL. (2010) Calcium-permeable AMPA receptor dynamics mediate fear memory erasure. Science. 330(6007): 1108-12.
PubMed Reference

Hayashi T, Thomas G, Huganir RL. (2009) Dual palmitoylation of NR2 subunits regulates NMDA receptor trafficking. Neuron. 64(2): 213-226.
PubMed Reference

Lin DT, Makino Y, Sharma K, Hayashi T, Neve R, Takamiya K, Huganir RL (2009) Regulation of AMPA receptor GluR1 subunit extrasynaptic insertion events by 4.1N, phosphorylation and palmitoylation. Nat. Neurosci. 12(7): 879-87.
PubMed Reference

Sia, G.-M., Beique, J.-C., Rumbaugh, G., Cho, R., Worley, P.F., Huganir, R.L. (2007) Interaction of the N-terminal domain of the AMPA receptor GluR4 subunit with the neuronal pentraxin NP1 mediates GluR4 synaptic recruitment. Neuron. 55(1): 87-102.
PubMed Reference

Steinberg, J.P., Takamiya, K., Shen, Y., Xia, J., Rubio, M.E., Yu, S., Jin, W., Thomas, G.M., Linden, D.J., Huganir, R.L. (2006) Targeted in vivo mutations of the AMPA receptor subunit GluR2 and its interacting protein PICK1 eliminate cerebellar long-term depression. Neuron. 49(6): 845-60.
PubMed Reference

Chung HJ, Steinberg JP, Huganir RL and Linden DJ. (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755.
PubMed Reference

Lee HK, Takamiya K, Han JS, Man H, Kim CH, Rumbaugh G, Yu S, Ding L, He C, Petralia RS, Wenthold RJ, Gallagher M, and Huganir RL. (2003) Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell. 112:631-642.
PubMed Reference

Lee HK, Barbarosie M, Kameyama K, Bear MF, and Huganir RL. (2000) Regulation of distinct AMPA receptor phosphorylation sites during bidirectional synaptic plasticity. Nature. 405:955-959.
PubMed Reference

Ehlers, M.D., Zhang, S., Bernhardt, J.P., and Huganir R.L. (1996) Inactivation of NMDA receptors by direct interaction of calmodulin with NR1 subunit. Cell 84:745-755.
Science Direct

Takanari Inoue, Ph.D.
jctinoue@jhmi.edu
443-287-7668

Synthetic cell biology: total synthesis of cellular functions such as neutrophil chemotaxis and ciliary mechano-sensation

Takanari Inoue
Associate Professor of Cell Biology
Johns Hopkins University School of Medicine

453 Rangos
Baltimore, MD 21205

Email: jctinoue@jhmi.edu
Office Phone: 410-614-8375
Lab Phone:

Visit my Lab Page

Program Description

Research Focus

Our research focuses on “synthetic cell biology” to dissect and reconstitute intricate signaling networks. In particular, we investigate positive-feedback mechanisms underlying the initiation of neutrophil chemotaxis (known as a symmetry breaking process), as well as spatio-temporally dynamic information processing at various compartments in living cells. In parallel, our lab also tries to understand how cell morphology affects biochemical functions. Ultimately, we will generate completely orthogonal nano-machinery in artificial cells that can achieve existing, and even novel, cellular functions.

Message for BCMB Students

Our research is conducted in an open lab style building which embodies a multidisciplinary research. Students who are enthusiastic about learning diverse disciplines, developing innovative techniques and challenging fundamental biological problems would enjoy the environment. Students are lways welcomed to our lab for discussions about potential research projects.

Recent Publications

Miyamoto T., Rho E, Sample V., Akano H., Magari M., Ueno T., Chen M., Tokumitsu H., Zhang J., and Inoue T. “Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators” Cell Reports 11, 657-670 (2015)
PubMed Reference

Lin B., Yin T., Wu Y.I., Inoue T. and Levchenko A. “Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration” Nature Communications 6, 6619 (2015)
Note: Press release: “A digital field guide to cancer cells”
PubMed Reference

Adams P.J., Johny M.B., Dick I.E., Inoue T., and Yue D.T., “Apocalmodulin itself promotes ion channel opening and Ca2+ regulation” Cell 159 (3), 608-622 (2014)
PubMed Reference

Razavi S., Su S. and Inoue T. “Cellular Signaling Circuits Interfaced with Synthetic, Post-Translational, Negating Boolean Logic Devices” ACS Synthetic Biology 3 (9), 676-685 (2014)
Note: “Introducing Authors” ACS Synthetic Biology
PubMed Reference

Onuma H., Komatsu T., Arita M., Hanaoka K., Ueno T., Terai T., Nagano T., and Inoue T. "Rapidly rendering cells phagocytic through a cell-surface display technique and concurrent Rac activation" Science Signaling, 7, rs4, 1-7 (2014)
Note: “Cover Story” Science Signaling, “Turning cells into garbage collector” Editor’s Choice in Science, Interviewed and broadcasted by FOX45 NEWS (https://drive.google.com/file/d/0B-wUSlcBaaEsSmpmQmhIblMzTXc/view?usp=sharing), Interview for “Thought Leaders” series by Medical-News, Press release: MOLECULAR “EAT NOW” SIGNAL MAKES CELLS DEVOUR DYING NEIGHBORS, “Training Cells to Devour Dying Neighbors” highlighted in NIGMS Biomedical Beat Blog, Highlighted in 5th Annual Henrietta Lacks Memorial Lecture, “Designer Immune Cells” highlighted in 2014 Research Highlights from Johns Hopkins Medicine (https://www.youtube.com/watch?v=Lb58GUNP5Qk)
PubMed Reference

Suarez A, Ueno T, Huebner R, McCaffery JM, and Inoue T “Bin/Amphiphysin/Rvs (BAR) family members bend membranes in cells” Scientific Reports 4, (4693) 1-6 (2014)
PubMed Reference

Kobayashi T, Kim S, Lin YC, Inoue T, and Dynlacht BD. “CP110-interacting proteins, Talpid3 and Cep290, play overlapping and distinct roles in cilia assembly” Journal of Cell Biology 204, 215-229 (2014)
PubMed Reference

Su S, Phua SC, DeRose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda . and Inoue T. “Genetically encoded calcium indicator illuminates calcium dynamics in cilia” Nature Methods 10, 1105-1107 (2013)Note: “Author File” Nature Methods
PubMed Reference

Thevathasa, JV, Tan E, Hui Z, Lin YC, Li Y. Inoue T and Fivaz M. “Local positive feedback from PI3K to Ras drives cell polarization and migration” Molecular Biology of the Cell (doi:10.1091/mbc.E12-12-0905)
PubMed Reference

Lin YC, Su S. and Inoue T. “Visualizing Molecular Diffusion through Passive Permeability Barriers in Cells: Conventional and Novel Approaches“ Current Opinion in Chemical Biology 2013 (doi:10.1016/j.cbpa.2013.04.027)
PubMed Reference

Lin YC, Liu TY, Razavi S and Inoue T. “Rapidly Reversible Manipulation of Molecular Activities Using Dual Chemical Dimerizers” Angewandte Chemie 52, 6450 (2013)
PubMed Reference

Lin YC, Niewiadomski P, Lin B, Nakamura H, Phua SC, Jiao J, Levchenko A, Inoue T, Rohatgi T, and Inoue T. “Chemically-inducible diffusion trap reveals molecular sieve-like barrier at primary cilia“ Nature Chemical Biology 9, 437 (2013)
PubMed Reference

Note: “Research on cilia heats up: Implications for hearing vision loss and kidney disease” Press release, Featured in “ChemBioVault”, “New cilia research could have implications for kidney disease” (American Society of Nephrology, In The Loop), “New clues about molecular composition of cilia” MIGMS Latest News, “Cilia Admit Large Cytosolic Proteins”, Kidney News, “Signaling: Shifting at Ciliary Base” News & Views, Nature Chemical Biology

DeRose R, Miyamoto T and Inoue T. “Manipulating signaling at will: chemically-inducible dimerization (CID) techniques resolve problems in cell biology“ Pfülgers Archiv 465, 409-417 (2013)
PubMed Reference

Miyamoto T, Razavi S, DeRose R, and Inoue T. “Synthesizing Biomolecule-based Boolean Logic Gates” ACS Synthetic Biology 2, (2), 72-82 (2013)
Note: “Podcast” (ACS Synthetic Biology)
PubMed Reference

Landon Stuart King, M.D.
lsking@jhmi.edu
443-287-3343

Gregg Semenza, M.D., Ph.D.

Molecular mechanisms of oxygen homeostasis; Gene and stem cell therapy for ischemic cardiovascular disease; Role of HIF-1 in cancer; Protection of the heart against ischemia-reperfusion injury

Paul Watkins, M.D., Ph.D.
watkins@kennedykrieger.org
443-923-2754

Fatty Acid Activation Enzymes

Paul Watkins
Professor, JA of Neurology
Johns Hopkins University School of Medicine

707 N. Broadway, Rm 400G
Baltimore, MD 21205

Email: watkins@kennedykrieger.org
Office Phone: 410-502-8279
Lab Phone: 443-923-2754

Visit my Lab Page

Program Description

My laboratory's primary interest is fatty acid metabolism. Fatty acids, from the shortest 2-carbon acid to the very long-chain fatty acids (VLCFA) containing 26 or more carbons, must be activated to their coenzyme A thioester before participating in metabolic processes. Using bioinformatics approaches and data from genome sequencing projects, we determined that humans and mice have ~26 different enzymes that catalyze this reaction—the acyl-CoA synthetases. Conserved motifs in their amino acid sequences have allowed us to classify them into families of related proteins. Although we know a bit about the fatty acid chain-length specificity of the acyl-CoA synthtases, their tissue expression patterns, and perhaps their subcellular location, we know little about the precise function of each enzyme. Our hypothesis is that the ACSs not only activate fatty acids but also direct them toward a specific metabolic fate in a specific compartment of the cell.

Two “families” of acyl-CoA synthetases are the subjects of current research. One is the “very long-chain” acyl-CoA synthetase family, which contains 6 members. We found that the expression of one member of this family is significantly increased in human brain and lung cancer. Knocking down this enzyme in human glioblastoma cells decreases their malignant behavior in vitro, and decreases their ability to produce tumors in mice. The role of this enzyme in cancer cells with respect to lipid metabolism and signal transduction pathways is an area of active investigation.

The “bubblegum” family, named for a fruitfly mutant, has 2 members. One member is of interest because the enzyme is only found in neurons and in steroidogenic cells of the adrenal glands and testes, precisely the cell types affected pathologically in the peroxisomal disease X-linked adrenoleukodystrophy (ALD). New studies have begun to shed light on the regulation of this enzyme, and suggest a role for it in the synthesis of sphingolipids and in GABAA receptor function. The second “bubblegum” family member is only found in testicular Sertoli cells and in large motorneurons of the brainstem. We are investigating a possible role for this enzyme in male fertility, and perhaps in amyotrophic lateral sclerosis (Lou Gherig’s disease).

Recent Publications

Pei Z, Sun P, Huang P, Lal B, Laterra J, and Watkins PA (2009) ACSVL3 depletion decreases the malignant phenotype of human gliomas. Cancer Res. 69, 9175-9182.
PubMed Abstract

Watkins PA (2008) Very long-chain acyl-CoA synthetases. J. Biol. Chem. 283, 1773-1777.
PubMed Abstract

Jia Z, Moulson CL, Pei Z, Miner JH, and Watkins PA (2007) FATP4 is the principal very long-chain fatty acyl-CoA synthetase in skin fibroblasts. J. Biol. Chem. 282, 20573-20583.
PubMed Abstract

Watkins PA, Maiguel D, and Jia Z (2007) Evidence for 26 distinct acyl-CoA synthetase genes in the human genome. J. Lipid Res. 48, 2736-2750.
PubMed Abstract

Pei Z, Jia Z, and Watkins PA (2006) The second member of the human and murine “bubblegum” family is a testis- and brainstem-specific acyl-CoA synthetase. J. Biol. Chem. 281, 6632-6641.
PubMed Abstract

Rui Zhou,

727-767-2166

Peter Agre, M.D.
pagre@jhu.edu
443-287-8745

Directs the Johns Hopkins Malaria Research Institute: lab studies in Baltimore and field work in Africa

Peter Devreotes, Ph.D.
pnd@jhmi.edu
410-955-3225

Genetic analysis of chemotaxis eukaryotic cells

Peter Devreotes
Professor and Director of Cell Biology
Johns Hopkins University School of Medicine

725 N. Wolfe St., 114 WBSB
Baltimore, MD 21205

Email: pnd@jhmi.edu
Office Phone: 410-614-9461
Lab Phone: 410-955-4699

Visit my Lab Page

Program Description

Recent Publications

PubMed Reference

Xiong Y, Huang CH, Iglesias PA., and Devreotes PN. 2010 Cells navigate with a local-excitation, global-inhibition-biased excitable network. PNAS, 107:17079-17086.

PubMed Reference

Janetopoulos C, Borleis J, Vazquez F, Iijima M, and Devreotes PN. 2005 Temporal and spatial regulation of phosphoinositide signaling mediates cytokinesis. Dev. Cell 8:467-477.

PubMed Reference

Iijima M, Huang E, and Devreotes PN. 2002 Temporal and spatial regulation of chemotaxis. Dev. Cell 3, 469-478.

PubMed Reference

Yolanda M. Fortenberry, Ph.D.
yforten1@jhmi.edu
410-614-0285

Molecular mapping, Biochemistry, Regulation of tumor cells, Thrombosis, Hemostasis

Richard Huganir, Ph.D.
rhuganir@jhmi.edu
410-955-4050

Regulation of Neurotransmitter Receptors and Brain Function in Health and Disease

Richard Huganir
Professor and Director of Neuroscience
Johns Hopkins University School of Medicine

725 N. Wolfe St.
Baltimore, MD 21205

Email: rhuganir@jhmi.edu
Office Phone: (410) 955-0877
Lab Phone:

Visit my Lab Page

Program Description

Recent Publications

Volk LJ, Bachman JL, Johnson R, Yu Y, Huganir,RL. (2013) PKMz is not required for hippocampal synaptic plasticity, learning and memory. Nature. 493(7432): 420-3.
PubMed Reference

Thomas GM, Hayashi T, Chiu SL, Chen CM, Huganir RL. (2012) Palmitoylation by DHHC5/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking. Neuron. 73(3):482-96.
PubMed Reference

Clem R, Huganir RL. (2010) Calcium-permeable AMPA receptor dynamics mediate fear memory erasure. Science. 330(6007): 1108-12.
PubMed Reference

Hayashi T, Thomas G, Huganir RL. (2009) Dual palmitoylation of NR2 subunits regulates NMDA receptor trafficking. Neuron. 64(2): 213-226.
PubMed Reference

Chung HJ, Steinberg JP, Huganir RL and Linden DJ. (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755.
PubMed Reference

Ehlers, M.D., Zhang, S., Bernhardt, J.P., and Huganir R.L. (1996) Inactivation of NMDA receptors by direct interaction of calmodulin with NR1 subunit. Cell 84:745-755.
Science Direct

Takanari Inoue, Ph.D.
jctinoue@jhmi.edu
443-287-7668

Synthetic cell biology: total synthesis of cellular functions such as neutrophil chemotaxis and ciliary mechano-sensation

Takanari Inoue
Associate Professor of Cell Biology
Johns Hopkins University School of Medicine

453 Rangos
Baltimore, MD 21205

Email: jctinoue@jhmi.edu
Office Phone: 410-614-8375
Lab Phone:

Visit my Lab Page

Program Description

Research Focus

Message for BCMB Students

Recent Publications

Landon Stuart King, M.D.
lsking@jhmi.edu
443-287-3343

Gregg Semenza, M.D., Ph.D.

Molecular mechanisms of oxygen homeostasis; Gene and stem cell therapy for ischemic cardiovascular disease; Role of HIF-1 in cancer; Protection of the heart against ischemia-reperfusion injury

Paul Watkins, M.D., Ph.D.
watkins@kennedykrieger.org
443-923-2754

Fatty Acid Activation Enzymes

Paul Watkins
Professor, JA of Neurology
Johns Hopkins University School of Medicine

707 N. Broadway, Rm 400G
Baltimore, MD 21205

Email: watkins@kennedykrieger.org
Office Phone: 410-502-8279
Lab Phone: 443-923-2754

Visit my Lab Page

Program Description

Recent Publications

Pei Z, Sun P, Huang P, Lal B, Laterra J, and Watkins PA (2009) ACSVL3 depletion decreases the malignant phenotype of human gliomas. Cancer Res. 69, 9175-9182.
PubMed Abstract

Watkins PA (2008) Very long-chain acyl-CoA synthetases. J. Biol. Chem. 283, 1773-1777.
PubMed Abstract

Rui Zhou,

727-767-2166

Backup_Secondary-Appointments // <![CDATA[ _spBodyOnLoadFunctionNames.push("setupPageDescriptionCallout"); // ]]>

Peter AgreProfessor of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Peter DevreotesProfessor and Director of Cell Biology Johns Hopkins University School of Medicine

Program Description

Recent Publications

Yolanda M. FortenberryAssistant Professor of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Richard HuganirProfessor and Director of Neuroscience Johns Hopkins University School of Medicine

Program Description

Recent Publications

Takanari InoueAssociate Professor of Cell Biology Johns Hopkins University School of Medicine

Program Description

Recent Publications

Landon Stuart KingProfessor of Medicine of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Gregg SemenzaProfessor of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Paul WatkinsProfessor, JA of Neurology Johns Hopkins University School of Medicine

Program Description

Recent Publications

Rui ZhouAssistant Professor of Medicine Johns Hopkins University School of Medicine

Program Description

Recent Publications

Peter AgreProfessor of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Peter DevreotesProfessor and Director of Cell Biology Johns Hopkins University School of Medicine

Program Description

Recent Publications

Yolanda M. FortenberryAssistant Professor of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Richard HuganirProfessor and Director of Neuroscience Johns Hopkins University School of Medicine

Program Description

Recent Publications

Takanari InoueAssociate Professor of Cell Biology Johns Hopkins University School of Medicine

Program Description

Recent Publications

Landon Stuart KingProfessor of Medicine of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Gregg SemenzaProfessor of Johns Hopkins University School of Medicine

Program Description

Recent Publications

Paul WatkinsProfessor, JA of Neurology Johns Hopkins University School of Medicine

Program Description

Recent Publications

Rui ZhouAssistant Professor of Medicine Johns Hopkins University School of Medicine

Program Description

Recent Publications

Backup_Secondary-Appointments

Peter Agre
Professor of
Johns Hopkins University School of Medicine

Peter Devreotes
Professor and Director of Cell Biology
Johns Hopkins University School of Medicine

Yolanda M. Fortenberry
Assistant Professor of
Johns Hopkins University School of Medicine

Richard Huganir
Professor and Director of Neuroscience
Johns Hopkins University School of Medicine

Takanari Inoue
Associate Professor of Cell Biology
Johns Hopkins University School of Medicine

Landon Stuart King
Professor of Medicine of
Johns Hopkins University School of Medicine

Gregg Semenza
Professor of
Johns Hopkins University School of Medicine

Paul Watkins
Professor, JA of Neurology
Johns Hopkins University School of Medicine

Rui Zhou
Assistant Professor of Medicine
Johns Hopkins University School of Medicine

Peter Agre
Professor of
Johns Hopkins University School of Medicine

Peter Devreotes
Professor and Director of Cell Biology
Johns Hopkins University School of Medicine

Yolanda M. Fortenberry
Assistant Professor of
Johns Hopkins University School of Medicine

Richard Huganir
Professor and Director of Neuroscience
Johns Hopkins University School of Medicine

Takanari Inoue
Associate Professor of Cell Biology
Johns Hopkins University School of Medicine

Landon Stuart King
Professor of Medicine of
Johns Hopkins University School of Medicine

Gregg Semenza
Professor of
Johns Hopkins University School of Medicine

Paul Watkins
Professor, JA of Neurology
Johns Hopkins University School of Medicine

Rui Zhou
Assistant Professor of Medicine
Johns Hopkins University School of Medicine